Rabbit anti-Horse IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN101452

Supplier Product No.: 608-4503

Quick Overview for Rabbit anti-Horse IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) (ABIN101452)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Horse

Host: Rabbit

Supplier Product No.: 608-4503

Supplier: Rockland

Purpose: Horse IgG Fc Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(c) and Horse Serum.  No reaction was observed against Horse IgG F(ab).

Characteristics: Anti-Horse IgG Alkaline Phosphatase Antibody generated in rabbit detects horse IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Horse IgG F(c) fragment

Application Details

Application Notes: Application Note: Anti-Horse IgG F(c) Alkaline Phosphatase has been tested by ELISA. This product has been assayed against 1.0 μg of Horse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.  Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Receptors bind the Fc portion of horse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.